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The in vivo biotinylation and purification strategy outlined here provides a basis to determine binding kinetics using a multitude of methods including surface plasmon resonance (Biacore, GE Life Sciences) or bio-layer interferometry (Octet, Pall ForteBio)

PLoS One 15:e0240448

When you're engaged in serious research, every single variable matters

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Vitamins that often do not need lab tests first The key phrase here is often do not, not never. There are always exceptions

Phosphorylated Smad2 associates with STAT3 to facilitate Th17 cell polarization, whereas unphosphorylated Smad3 interacts with STAT3 to inhibit Th17 cell differentiation