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for 10 min, 25 L of agonist was dispensed into the well using a FlexStation III microplate reader (Molecular Devices), and the intracellular calcium change was recorded at an excitation wavelength of 485 nm and an emission wavelength of 525 nm

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Retatrutide is an investigational triple receptor agonist targeting GLP-1, GIP, and glucagon receptors, not yet approved by the MHRA or EMA, with Phase 3 trials ongoing

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