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EGFR/MAPK signaling pathway acts as a potential therapeutic target for sulforaphane-rescued heart tube malformation induced by various concentrations of PhIP exposure

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The 29 gauge represents optimal balance between ease of insertion and flow rate, thinner needles (31 gauge) hurt less but inject more slowly, while thicker needles (27 gauge) inject faster but cause more tissue trauma

doi:10.1007/BF02978833

The microplate was then incubated at 22 C for 10 minutes

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