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5 Bajpai J, Kapoor A, Jalali R, Gounder MM

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Non-adherent cells were then removed, and the adherent cells were differentiated into macrophages using recombinant good manufacturing practice (GMP) macrophage-colony stimulating factor (M-CSF: 10 ng/mL

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Cells with less than 1000 genes or more than 20,000 genes, and cells with more than 10% (fetus) or 50% (pediatric and adult) of mitochondrial transcripts were considered as ambient RNA or doublet and excluded

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