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This is a fundamental limitation of the enterprise: the arithmetic is exact, but its inputs are uncertain, so an exact calculation on an uncertain input yields an uncertain result dressed up in false precision

Cys-SH can be transformed to 3-mercaptopyruvate (3-MP) in reaction catalyzed by PLP-dependent cysteine aminotransferase (CAT)

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Once you puncture the rubber stopper, you've introduced potential contamination