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10.3109/15412555.2010.510160 18 BhagatM.SinghA.BazziT.GreenJ

coli is a single, non-glycosylated polypeptide chain (a.a 22-115) containing 104 amino acids including a 10 a.a N-terminal His tag

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In aqueous solutions, especially at physiological temperatures (37C), L-glutamine can spontaneously break down into ammonia and pyrrolidone carboxylic acid (Figure 1)