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However, this mechanism remains an area of ongoing research rather than established fact

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Buffer was then collected and centrifuged at 3,000 g for 10 min at 4 C, and 500 L of supernatant was used to measure insulin levels using the Mouse Insulin ELISA Kit

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After treatment with IGF-1, IGF-1R levels increased in membrane fraction of all the cell lysates compared to cytosolic fractions indicating IGF-1R migration to the membrane (Fig