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The assessment of gene expression by qPCR was performed as previously described [27, 28]

If you have any serious side effects, including, itching, yellow eyes or skin, dark urine, loss of appetite, severe or continuous abdominal pain, hives, vomiting, chest pain, rectal bleeding, constipation that lasts more than 7 days, difficulty swallowing or breathing, or you are taking medicine for seizures and your seizures happen more often or get worse, stop using the medication and talk to your healthcare provider

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Gastrointestinal issues occur when the stomach cannot function properly

(2007) Changes in 24-h area-under-the-curve ghrelin values following diet-induced weight loss are associated with loss of fat-free mass, but not with changes in fat mass, insulin levels or insulin sensitivity

Only trained professionals should handle it following standard laboratory safety protocols