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Be sure to consult your doctor or health professional if youre considering adding milk thistle into your diet but have underlying health conditions

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To ventralize the progenitors 1M SAG (Merck) and 1M Purmorphamine (Sigma) were added between day 2 and day 8

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The supernatant was discarded, and the pellet resuspended in cold PBS + 1% Triton X-100 plus protease and phosphatase inhibitor cocktails, transferred to an Eppendorf tube and allowed to rotate for 30 minutes at 4C