PMXS_IRES_BLAST_Hu_SLC25A39_D226A mutant
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Best crystals grew in 50 mM HEPES, pH 7.0, 4% PEG 8000, 100 mM NaCl and were cryoprotected by transferring to a solution containing 25% glycerol as cryoprotectant along with all the components of protein and crystallization mother liquor
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Total RNA was harvested from monolayer and pellet cultures at different time points using Qiazol (Qiagen, Germantown, MD, United States) and purified with RNeasy Plus Universal Mini Kit (Qiagen, Cat
TAS participated in the recruitment of patients, design and application of control diet and HAD, carried out the nutritional counseling and oxidative stress determinations