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When confluent, HAECs were shifted to ALI culture with 50 L apical media for 8 days to achieve full differentiation with and without IL-13 (10 ng/mL) stimulation (R&D Systems, 213-ILB)

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GSSG concentration is then measured by spectrophotometry with the GSH recycling method, on the basis of conversion of GSSG to GSH by glutathione reductase and NADPH and reaction with 5,5-dithiobis-(2-nitrobenzoic acid)

2.8 In vitro evaluations of drug release The drug release was evaluated using the dialysis membrane method

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Proestrus is defined by large proportions of small nucleated epithelial cells (SNEs), but low numbers of leukocytes (LEUs), large nucleated epithelial cells (LNEs), and non-nucleated keratinized epithelial cells (AKEs)