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Chemokine (CC motif) ligand-2 (CCL2) and oxidative stress markers in recurrent pregnancy loss and repeated implantation failure

After that, cells were incubated with the secondary antibody (Alexa Fluor 488 donkey anti-goat, 1:500 in 3% BSA-0.1% triton X-100 PBS

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Usually temporary and mild to moderate

Shaking creates violent physical forces inside the vial that tear apart the peptide structure

Allow the vial to sit for 12 minutes