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Tissue whole-mounts or membrane inserts were then blocked for 1 h in blocking buffer (0.5% BSA, 0.25% fish skin gelatin, 1% Triton X-100 and 10% donkey serum) in PHEM buffer (60 mM PIPES, 25 mM HEPES, 10 mM EGTA and 4 mM MgSO 4 7H 2 O)

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Both before bed, separate syringes

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Winstrol (Stanozolol) : Increases vascularity and muscle hardness without water retention (Hartgens & Kuipers, Sports Medicine , 2004)