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Detergent was removed by adding Bio-Beads SM-2 (100 mg/ml) (Bio-Rad, 1523920) and rotating for 2 hours at room temperature followed by five additional rounds at 4C every two hours

However, precisely mapping VDR distribution in the brain is challenging

Zinc is essential for the immune system, wound healing, and the formation of DNA

The olfactory region comprises just 35% of the total nasal cavity surface area yet is the most critical anatomical region regarding IN drug administration [17]

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The primary ESCs were obtained according to previously described methods [26, 27]