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Live/dead staining assay Raw 264.7 cells were co-cultured with LPS at a density of 7 10 4 cells per well in a 12-well plate for 24 h

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You may hear a faint hiss as you puncture the stopper for the first time

J Neural Transm (Vienna) 129:10771089 Kuratsune H, Watanabe Y, Yamaguti K, Jacobsson G, Takahashi M, Machii T, Onoe H, Onoe K, Matsumura K, Valind S, Kitani T, Langstrom B (1997) High uptake of [2-11c]acetyl-l-carnitine into the brain: a pet study

Use a 45-degree angle with a skin pinch when subcutaneous fat at the site is limited