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(B,D) The cultured middle turn cochleae were treated with peroxynitrite (100 M) alone, or cotreated with ebselen (30 M) for 48 h

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Matek, D., Matek, I., Staresinic, E., Japjec, M., Bojanic, I., Blagaic, A

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After incubation, cells were washed with PBS twice, re-suspended, and immediately submitted to flow cytometric analysis using a BD Accuri C6 Plus flow cytometer (Becton Dickinson, Mountain View, CA, USA)