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Samples with detergent were mixed with detergent (final 0.005% (v/v) Tween-20) just before vitrification
As for PBN, the reaction with superoxide is quite slow (thus requiring large doses to be efficient in vivo ) (Day, 2004)
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Example 4: sepsis and its complications GSH as a regulatory molecule ARDS is one of the most serious complications in critically ill septic patients
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(PubMed) Zhang Y, Jiang W, Xie Z, Wu W, Zhang D
After being fixed with 99% ethanol and overnight incubation, cells were washed and Rnase (Sigma-Aldrich), and propidium iodide (Sigma-Aldrich) was added to the cells
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