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PC cells were harvested after trypsinization, followed by centrifugation and then fixed at 4 C overnight in Karnovskys fixative (2% paraformaldehyde and 5% glutaraldehyde in 0.1 M cacodylate, pH 7.4)

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Anti-ubiquitinated myeloperoxidase antibodies in SLE sera enhance cytokine production by macrophages upon NET stimulation, suggesting a role for extracellular ubiquitin in lupus-related inflammation