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By conducting KEGG metabolic pathway enrichment analysis on the differential metabolites of each group, it can be observed that a high-fat diet affects the biosynthesis of phenylalanine, tyrosine, and tryptophan, taurine and hypotaurine metabolism, phenylalanine metabolism, and arachidonic acid metabolism in mice

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Data showed that both TNF- and IL-1 levels were increased with LPS treatment, while co-treatment with exendin-4 significantly reduced their secretion into the media (Figure 4)