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On day 2, the medium was replaced with DMEM supplemented with 10% FBS and 10 g/mL insulin, refreshed every 2 days

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Female sex, age over 40, family history of gallstones, and conditions like insulin resistance or metabolic syndrome all increase susceptibility

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Dissecting cellular mechanisms of Long-Chain Acylcarnitines-Driven cardiotoxicity: disturbance of calcium homeostasis, activation of Ca2+-Dependent phospholipases, and mitochondrial energetics collapse