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Samples were separated by SDS-PAGE and subjected to immunoblotting using a primary anti-acetyl-lysine antibody (abcam, ab21623, dilution 1:10,000) in combination with a secondary HRP-coupled anti-rabbit IgG antibody (abcam, ab6721, dilution: 1:10,000)

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They shorten every time a cell divides, until they become too short to allow proper cell division

PMID: 40628573 ) Schol J, Huang IH, Carbone F, Fernandez LMB, Gourcerol G, Ho V, Kohn G, Lacy BE, Colombo AL, Miwa H, Moshiree B, Nguyen L, OGrady G, Siah KTH, Stanghellini V, Tack J (2025) Rome Foundation and international neurogastroenterology and motility societies consensus on idiopathic gastroparesis

Krawczyk M, Liebe R, Maier IB, Engstler AJ, Lammert F, Bergheim I