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For bacterial diversity analysis, the V3V4 (or V4V5) variable regions of the 16S rRNA genes were amplified with universal primers 343F (5-TACGGRAGGCAGCAG-3) and 798R (5-AGGGTATCTAATCCT-3) 1 for the V3V4 regions, or 515F (5-GTGCCAGCMGCCGCGG-3) and 907R (5-CCGTCAATTCMTTTRAGTTT-3) 2 for V4V5 regions]

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