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The resin was washed with 10 resin volumes of the washing buffer (20 mM Hepes pH 7.5, 400 mM NaCl, 20 mM Imidazole) and eluted with the elution buffer (20 mM Hepes pH 7.5, 400 mM NaCl, 400 mM Imidazole)

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Then, you might need them every two to four weeks after that