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The Free GSH concentration in the sample is calculated from the difference between the Total GSH determined and the GSH generated from oxidized glutathione for the 2-vinylpyridine-treated samples

The process stays the same, just repeated

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the focus will be different, so these effects might not be as potent (or immediate), but they still may occur

Because DSIP research is largely historical and not industrially funded, it lacks standardized research protocols, making comparison across studies difficult