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Cells were loaded with 2 M BODIPY 581/591 C11 (Thermo Fisher Scientific) for 30 min and harvested for analysis on an Attune acoustic flow cytometer (Applied Biosystems)

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After structural refinement of the X-ray structure of TGR into the cryo-EM map obtained at 3.6 resolution (as estimated by the gold-standard Fourier shell correlation at 0.143 Supplementary Fig

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