M.BedlackR
Assuming you followed the above advice and used an appropriate binding buffer and your protein isnt huge, then a likely reason for this is that the expression yield is much lower than you expected
A 2019 study found that vitamin B12 supplementation can be associated with an increased risk of colon cancer in older adults when taken at high doses
Vitamin B12 is also important for DNA synthesis and cell maturation
Neuroscience 504 , 1020 (2022)
10.1016/j.phymed.2018.11.020 Summary Keywords acetaminophen, liver injury, natural products, oxidative stress, Nrf2 Citation Liao J, Lu Q, Li Z, Li J, Zhao Q and Li J (2023) Acetaminophen-induced liver injury: Molecular mechanism and treatments from natural products