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Blood-brain barrier breakdown involves four distinct stages of vascular damage in various models of experimental focal cerebral ischemia

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After collagen treatment of the lesion-containing material and sieving, the washed cells were layered over a five-layer discontinuous Nycodenz gradient of 0.5, 1.0, 5.0, 10 and 30% (w/v) Nycodenz and centrifuged at 1200 g for 15 min at 10C [13,14]

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