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Protein expression was induced by addition of 0.1 mM IPTG and cultured for an additional 16 18 hrs at 25 C

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Median age at onset of CVS was 4.92 years [IQR 6.86], median age at diagnosis was 9.81 years [IQR 6.18] and median age at treatment was 10.00 years [IQR 7.00]

The one-on-one consultations, tailored weight loss plan, and regular check-ins kept me accountable and motivated throughout my journey

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