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50 L of 100 mM/L 3,5 dichloro-2-hydroxy benzene sulfonate in phosphate buffer (pH 7) was used as a chromogen, and 500 L of 4-amino antipyrine peroxidase preservatives were added to 50 L of the sample or standard, and 500 L of chromogen and 500 L deionized water were added to 50 L of the blank sample

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